Oral Presentations: Abstracts of the 13th Red Lara Taller General, Buenos Aires, Argentina, 26-28 April 2017

s of the 13th Red Lara Taller General, Buenos Aires, Argentina, 26-28 April 2017 O-02. FSH dose to stimulate different patient’ ages: when less is more E. Borges Jr.1,2, B.F. Zanetti2, A.S. Setti2, D.P.A.F. Braga1,3, R.C.S. Figueira1, A. Iaconelli Jr.1 1Fertility – Medical Group, São Paulo, SP – Brazil 2Instituto Sapientiae – Centro de Estudos e Pesquisa em Reprodução Humana Assistida, São Paulo, SP Brazil 3Disciplina de Urologia, Área de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo. UNIFESP Objective: To determine the effect of FSH doses on intracytoplasmic sperm injection (ICSI) outcomes according to the age of the patient. Methods: Patients undergoing controlled ovarian stimulation (COS) for ICSI cycles in a university-affiliated in vitro fertilization center were split into age groups: ≤35 y.o. (n=1523); >35 and ≤38 y.o. (n=652); >38 and ≤40 y.o. (n=332); and >40 y.o. (n=370). The effect of FSH dose on COS, laboratorial and clinical outomes was determined by linear regression models. Results: The FSH dose didn’t affect the ovarian response in terms of total number of follicles, retrieved oocytes and mature oocytes within the age groups, but it was observed that the lower the age, the lower the FSH dose is needed per oocyte retrieved. In the group of patients ≤35 y.o., it was also noted a positive effect of FSH dose on oocyte yield. Despite that, for patients ≤38 y.o. it was verified a negative effect of FSH dose on embryo quality and blastocyst formation rate, and also an increase in cycle’s cancelation rate. In patients ≥ 39 y.o., there were no effects of the FSH doses on the analysed variables. Conclusion: The ovarian stimulation with high doses of FSH is not recommended in younger women (≤38 y.o.), once it was observed a decrease in embryo quality and an increase in cycle ́s cancelation rate. Mild ovarian stimulation protocols may be more appropriate; however it may not be applicable for women in advanced age, since its necessary a higher FSH dose for oocyte retrieval in these patients. O-03. The adverse effect of overweight in Assisted Reproduction Outcomes A.V. Sampo1, C. Palena1, L. Ganzer1, V. Maccari1, G. Estofán1, M. Hernández1 1CIGOR – Centro Integral de Ginecología, Obstetricia y Reproducción. Córdoba, Argentina Objective: To assess the effect of Body Mass Index (BMI) on the results obtained in ICSI cycles. Methods: We studied 266 ICSI cycles realized between January 2014 and December 2016. Patients were grouped according to their BMI in: Normal (18.5-24.9), Overweight (25.029.9) and Obese (>30). We compared between the groups: antral follicles number, ovarian stimulation lenght, gonadotropin dose used, maximum oestradiol value, follicles developed /antral follicles, retrieved oocytes/developed follicles and mature/retrieved oocytes, normal fertilization rate, embryo achieved/ normal fertilized oocytes, clinical pregnancy and implantation rate. Kruskal-Wallis and Chi square test were used. P<0.05 was considered significant. Results: Normal, Overweight and Obese patients presented comparables values for antral follicles number (11.6±5.4, 12.5±5.5, 12.2±5.7), ovarian stimulation length (7.5±1.4, 7.6±1.1, 7.8±1.3) and gonadotropin dose used (2043±489, 1940±536, 2109±605). Obese patients had lower values of oestradiol (1560±610, 1511±635, 1190±466; P=0.018), developed follicles (81%, 76%, 70%; P<0.0001); and retrieved oocytes (91%, 90%, 84%; P=0.0017); and not significantly lower values of mature oocytes (82%, 82%, 77%; P=0.26). Groups had comparable fertilization rate (72%, 73%, 69%) and embryo achieved rate (67%, 63%, 72%). Normal group showed higher but not significant pregnancy and implantation rates (43%, 40%, 38%, P=0.53; and 33%, 26%, 23%; P=0.11), and significantly higher ongoing pregnancy rate (37%, 33%, 33%, P=0.042) Conclusion: Increased BMI patient had impaired ovarian response and pregnancy rate in ICSI cycles. 138 Abstracts of the 13th Red Lara Taller General, Buenos Aires, Argentina, 26-28 April 2017 JBRA Assist. Reprod. | v.21 | no2| Apr-May-Jun/ 2017 O-04. Can ovarian doublestimulation in the same menstrual cycle improve the results of IVF? M.C.A. Cardoso1, A. Evangelista1,2, C. Sartório1, G. Vaz1,2, C.L.V. Werneck1, F.M. Guimarães1, P.G. de Sá1,2, M.C. Erthal1 1Vida Centro de Fertilidade, Rio de Janeiro, Brazil 2Department of Gynecology of the State University of Rio de Janeiro (UERJ). Objetive: Evaluate the efficacy of the double-stimulation protocol over conventional ovarian stimulation in order to recover a more adequate number of oocytes to increase the number of embryos to be transferred or to be genetically analyzed. Methods: A retrospective and comparative study with 13 patients who underwent unsuccessful IVF cycles with a conventional antagonist ovarian stimulation protocol and repeated the attempt with a double stimulation protocol. The following variables were analyzed: number of oocytes collected, mature oocytes collected, fertilization rate, blastocyst rate, biopsied blastocyst rate and euploidy rate. Results: The double stimulation protocol had a significant higher number of oocytes collected (P=0.007) and mature oocytes to be injected (P=0.01). There was no statistical difference in fertilization (P=0.78) and blastocyst (P=0.59) rates. Conclusion: Double stimulation favors patients who are at risk of incurring several attempts of IVF in order to achieve pregnancy. O-05. Key performance indicators score (KPIs-score) based on clinical and laboratorial parameters can establish benchmarks for internal quality control in an ART program J.G. Franco Jr1,2, C.G. Petersen1,2, A.L. Mauri1,2, L.D. Vagnini2, A. Renzi2, B. Petersen2, M.C. Mattila1, V.A. Comar1, J. Ricci1, F. Dieamant1,2, J.B.A. Oliveira1,2, R.L.R. Baruffi1,2 1Center for Human Reproduction Prof. Franco Jr., Ribeirao Preto, Brazil. 2Paulista Center for Diagnosis, Research and Training, Ribeirao Preto, Brazil. Objective: KPIs have been employed for internal quality control (IQC) in ART. However, clinical KPIs (C-KPIs) such as age, AMH and number of oocytes collected are never added to laboratory KPIs (L-KPIs), such as fertilization rate and morphological quality of the embryos for analysis, even though the final endpoint is the evaluation of clinical pregnancy rates. This paper analyzed if a KPIs-score strategy with clinical and laboratorial parameters could be used to establish benchmarks for IQC in ART cycles. Methods: In this prospective cohort study, 280 patients (36.4±4.3years) underwent ART. The total KPIs-score was obtained by the analysis of age, AMH (AMH Gen II ELISA/ O-06. Morphological embryo selection: An elective Single Embryo Transfer proposal F.P. Déniz1, C. Encinas1, J. La Fuente1 1Embriovid, La Paz, Bolivia. Objective: To describe a patient selection methodology for elective single embryo transfer (eSET) eligibility, emphasizing the inclusion criteria and their results. Methods: Retrospective analysis. All cases carried out in a private clinic between June 2011 and December 2016, in La Paz, Bolivia (3600 meters above sea level). In 34 IVF/ICSI cycles, elective single embryo transfer was performed, all of them in blastocyst stage. Gardner’s blastocyst classification criteria were used. Between the two stages of the study (July 2015), each embryo grade implantation rate was recalculated, as a consequence, the inclusion criteria were expanded. Results: The clinical pregnancy rate of the 34 cases in the first transfer was 55.9% (19/34). Neither twin nor multiple pregnancies occurred. To date, the cumulative pregnancy rate is 64% [(19+3)/34]. The first stage comprised 2.56% (12/468) of patients who accessed the elective single embryo transfer program; the implantation rate was 58.3% (7/12). Whereas in the second stage, 14.29% (22/154) of patients were eligible, the implantation rate was 54.55 (12/22) respectively. Conclusion: The implementation of an eSET programme based on an in-depth morphological embryo assessment combined with the calculation of the implantation potential of each embryo grade are a suitable method to achieve acceptable clinical outcomes and to reduce multiple pregnancies in patients to whom two embryos are transferred. It is mandatory that every clinic must be aware of the implantation rate of each embryo grade in its own setting. pre-mixing modified, Beckman Coulter Inc.), number of metaphase-II oocytes, fertilization rates and morphological quality of the embryonic lot. Results: The total KPIs-score (C-KPIs + L-KPIs) was correlated with the presence or absence of clinical pregnancy. The relationship between the C-KPIs and L-KPIs scores was analyzed to establish quality standards, to increase the performance of clinical and laboratorial processes in ART. The logistic regression model (LRM), with respect to pregnancy and total KPIs-score (280 patients/102 clinical pregnancies), yielded an odds ratio of 1.24 (95%CI=1.16-1.32). There was also a significant difference (P<0.0001) with respect to the total KPIs-score mean value between the group of patients with clinical pregnancies (total KPIs-score=20.4±3.7) and the group without clinical pregnancies (total KPIs-score=15.9±5). Clinical pregnancy probabilities (CPP) can be obtained using the LRM (prediction key) with the total KPIs-score as a predictor variable. The mean C-KPIs and L-KPIs scores obtained in the pregnancy group were 11.9±2.9 and 8.5±1.7, respectively. Routinely, in all cases where the C-KPIs score was ≥9, after the procedure, the L-KPIs score obtained was ≤6, a revision of the laboratory procedure was performed to assess quality standards. Conclusion: This total KPIs-score could set up benchmarks for clinical pregnancy. Moreover, IQC can use C-KPIs and L-KPIs scores to detect problems in the clinical-laboratorial interface.